IGEM:Hong Kong UST 2014/2009/Notebook/ATP Concentration Effect to Ligation Speed/2014/06/03
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Tuesday, 3th of June 2014
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2. Inoculation of bacteria in broth:
E.coli SURE with RFP-PSB1C3 was obtained from four different colonies and inoculated to the 15 ml Falcon tube with 5 ml CAM LB broth. The tubes were incubated under 37ºC for 16 hours, 200 rpm.
The inoculated bacteria were named RFP-pSB1C3 1-4.