IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/23
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No colonies beyond control from last nights transformations
Today, we will create our very first biobrick vector with both the sense and intron parts. We will use parts digested yesterday, purify them, and ligate them together.
All the Antisense and intron lanes showed, though the last lane (PAL) was faint.
Gel Purification Nanodrop of gel purification had concentrations of:
There is some cause for concern for the lower concentrations, since these small numbers are outside of the range of accuracy.
Ligation of intron insert into Sense + V0120 PME was ligated into all backbones containing sense parts of the allergen panel (8 rxns), followed by PAL ligation into the same backbones. We plated two of each PAL ligation in case that there might be some increased chances of finding growth on plates if more than one plate is done for each ligation. We will check for colonies Saturday.
Genomic DNA extraction from Valencia Oranges
ODs Orange genomic DNA 1-1: 1.8ng/μL (260/280: .82) Orange genomic DNA 1-2: 1.1ng/μL (260/280: .68) Orange genomic DNA 2-1: 2.6ng/μL (260/280: .85) Orange genomic DNA 2-2: 2.1ng/μL (260/280: .89)
Mira/Brazz StrepII & Stop