IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/16
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More arabadopsis DNA arrived today. We are going to obtain more hpRNA parts and
Nanodrop of Miniprepped amiRNA Concentrations
Diagnostic Gel: Ladder, Bet1-6, LTP1-LTP4, Ladder; Ladder, LTP6, LTP.5 (1-6), Ladder
PCR of Arabidopsis Genomic DNA, attempt 2
File:716pcrnewdnafail.jpeg PCR failure - no product.
PCR using the newly extracted DNA was unsuccessful.
The degradation signal from the Austrians was successfully inoculated and colonies were found on the agar plates. Individual colonies from the plates were inoculated into tubes of 5 ml LB+Amp and placed in the shaking incubator overnight. (37°C)
Making Glyerol Stocks
Combined .5mL 80% glycerol with .5mL overnight cell culture of: Barnase 3 (number changed to Barnase 1 from miniprep onward), LacIN1, and Gal2.