- Colonies from the Miraculin/Brazzein-YFP-NOSt+STOP constructs were placed in 5 mL LB AMP media and left in the 37°C shaker.
- Plated bacteria was left for storage at 4°C
- PCRd pHAN.pdk, pKAN.pdk (they're the same 741bp pdk) using the values that worked in the gradient, got them in high ODs. Put them in box a2 rows 3-4.
Concentration of PCR products in ng/ul
Innoculating colonies from Friday's ligations
- Barnase, Gal4, LacIN
- 4 cc tubes each, 5mL LB+amp, set to shake at 37°C overnight