IGEM:Harvard/2006/vlau/Notebook/2006-6-13

Gel Electrophoresis w/ 2% Gel

1. Protocol

   - mixed 2g Ultrapure agarose w/ 100mL 1X TBE in plastic flask
   - heated for 1.5 min in microwave w/ top loosely screwed on
   - added 3μL EtBr
   - poured into gel frame and let set for 20 min
   - placed into running box and submerged w/ TBE
   - wells loaded
   - ran gel @ 130V, 45 min

2. Samples

   L. 1: 1kB ladder
   L. 2 and 3: DNA nanostructure
   L. 4 and 5: neg control w/ no oligonucleotides
   L. 6 and 7: neg control w/ no scaffold
   L. 8: 1 kB ladder
   loading dye: BTB dye 50% glycerol + 10X TBE
   ladder: 10μL + 1μL dye
   samples: 10μL + 1μL dye

3. Image

Transformation P. 2

1. Protocol

   - prepared 2 liquid cultures for each transformation
   - added 50μL of 5mg/mL amp to 5mL LB per culture
   - shook @ 180rpm, 37dC overnight