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Cyano continues! And you all thought we were dead...

To do

  • Chiba letter
  • Miniprep Nick's plates and sequence
  • Begin taking aliquots of M2-7 J04500+KaiC at time points
  • The other (less than 6) stage1 ligations

Verification of J04500+KaiC

Based on the (20!) sequencing results from way back in August (how is it freezing now...)I:

  • Created a file on MCB1001 (small room, left computer), ZS_J04500+kaiC which contains the expected plasmid we want
  • Aligned all 10 VF2's to the expected file
  • Tested fully one of them, M2-7

And we have the correct plasmid! First biobrick...