IGEM:Groningen/Notebook/iGEM 2011/2011/06/30

Project name

Main project page Previous entry      Next entry

30-6-11 Colony PCR of yesterday's transformants Taq 10× buffer: 20μl dNTPs 10mM: 4μl MgCl2: 12μl Forward Biobrick primer: 4μl Reverse Biobrick primer: 4μl Taq DNA polymerase: 1μl MQ water: 155μl PCR conditions: Preheated lid: 111°C Denaturation: 94°C for 10 min. Cycle 33×: denaturation: 94°C for 30s. annealing: 60°C for 30s. Extension: 72°C for 2,5 min. Final extension: 72°C for 10 min. Store infinite at 4°C Analyse on a 1% TBE agarose gel. Teammeeting at 3