IGEM:Groningen/Notebook/iGEM 2011/2011/06/21

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21-6-11 Digestion+ ligation PhybB product with taq polymerase Purify the PCR product with the High Pure PCR Purification kit from Roche Digestion mix: 18μl buffer 4.5μl EcoRI 4.5μl SpeI 49μl MQ water Vector mix: 18μl buffer 4.5μl EcoRI 4.5μl XbaI 49μl MQ water Ligation: 2μl T4 DNA ligase buffer 1μl T4 DNA ligase 7μl insert 3μl vector 7μl MQ water Ligate 2 hours at room temperature Ligate further overnight Helping with sponsoring Making researchplan