IGEM:Groningen/Notebook/iGEM 2011/2011/06/13

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13-6-11 Plasmid prep of the overnight cultures ND1000-nanodrop measurements: DNA concentrations are between 30 and 60 ng/μl Inoculating 2 erlenmeyers of 200ML , 1 for making competentcells DH5alpha and other one for TOP10 cells Also TOP10cells will be send to the iGEM Wageningen team. Also preheat other 2 200ml erlenmeyers Restriction analysis: Digestion test: 2μl FD buffer 0.5μl EcoRI 0.5μl SpeI 8μl MQ water 10μl + 10μl sample Digestion mix: 30μl FD buffer 7.5μl EcoRI 7.5μl PstI 120μl MW water Digestion mix: 30μl FD buffer 7.5μl XbaI 7.5μl SpeI 120μl MW water Work in Clone manager: check primers PhybB again