IGEM:Groningen/Notebook/iGEM 2011/2011/06/09

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09-06-11



Transformation
Add to 40μl competent cells 10μl ligation mixture
Incubate for 30 min on ice
Heat shock: 45s at 42 degrees
Incubate cells on ice for 2 min
Add 1ml LB medium+ 25mM glucose
Incubate cells for 1h/1.5h at 37 degrees
Spin the cells down, resuspend them in 100μl LB medium and plate 90μl and 10μl out on the plates
Put the plates in the stove at 37 degrees overnight

Meanwhile, prepare the plates with ampicillin and kanamycin
Work on Human practices and report+ researchplan
Teammeeting at 5!