IGEM:Groningen/Notebook/iGEM 2011/2011/06/06

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06-06-11 Plasmid Prep of the biobricks: DT, cI, LasRLVA, RFP,PBADaraC, RFP DT, PlasB, RBS-GFP-DT ND1000- nanodrop measurements: DT: 53.9 ng/μl cI: 64.1 ng/μl LasRLVA: 43.3 ng/μl RFP: 32.9 ng/μl PBADaraC: 53.2 ng/μl RFP DT: 53.3 ng/μl PlasB: 44.8 ng/μl RBS-GFP-DT: 52.5 ng/μl Samples were checked again with restriction analysis. Digestion mix: 14μl FD buffer 3.5μl EcoRI 3.5μl PstI 49μl MQ water 10μl mix+ 10μl DNA sample Analyse on a 1% TBE agarose gel Samples look alright, DT, cI, LasRLVA, RFP, PBADaraC, RFP DT, PlasB, RBS-GFP-DT were send for sequencing