IGEM:Cambridge/2008/Notebook/Voltage/2008/09/16

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Ligations

  • GluR0 + pSB4T5 or pSB3T5
  • B1002 + B1006

Gel

  • B1002 + B1006 ligation product run with GluR0 unsupercoiled
    • GluR0 was lightly irradiated to induce sing strand breaks to allow it to de-supercoil and act as a 4kb template.