IGEM:Cambridge/2008/Notebook/Voltage/2008/08/27

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osmY RBS

  • Primers containing RBS for osmY Biobrick were received
  • PCR was performed from biobrick J45250
  • Ligated into dephosphorylated vector backbone
  • Colonies transformed

Restriction Digest & Ligation Test

  • Various combinations of restriction digests & T4 phage genome ligation test.
  • All results positive :-)