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pSB4C5 & RBS

  • scPCR perfomed on 5 colonies of DB31 & TOP10 to determine whether they contain pSB4C5 and B0030 respectively.
  • Sucessful colonies grown up in LB, and pSB4C5 glycerol stocks were created.
  • Large quantities of pSB4C5 were also harvested.


  • Electroporation of cells with the Kdp-YFP backbone.
  • This failed (Even control, Jim!)