IGEM:Cambridge/2008/Notebook/Voltage/2008/08/19

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Ligation Diagnostic

  • Due to lack of kdp insert following scPCR of transformed colonies, the ligation procedure was tested, involving Kdp & Vector biobricks together and separately, dirty ligations, negative controls where buffer was added but no ligase etc.
  • Results of gel seemed inconclusive as hyperladder I ran very badly, but there seemed to be no DNA in the 'vector only lane' on both occasions.
  • Conclusion: investigate our supply of pSB4C5 vector.

Kdp

  • YPF biobrick plasmid used to provide vector backbone and a Kdp digest/ligation was attempted.