IGEM:Cambridge/2008/Notebook/Voltage/2008/08/06

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Kdp?

  • Transformants!
  • But... PCR showed that none contained the Kdp insert.
  • Gelled cut vector and extracted backbone band to use for ligation.
  • Ligated Kdp gene into backbone and transformed cells. Again.

Mutants

  • Grew up 5 mutants in LB, 1mM, 50mM and 200mM KCl.