IGEM:Cambridge/2008/Notebook/Voltage/2008/08/05

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Kdp? There were transformants from yesterday's transformation James celebrates apparent success After Biobrick PCR the gel showed short fragments, suggesting self ligation of the vector. Ellis realises the sad truth Repeated digest and ligation protocol from the weekend, with EcoR1 and Pst1 instead of Xba1 and Spe. Biobricks Extracted two GFP-only sequences (J04630 and I714062) from the registry, for use as reporters for osmY. PCR on these and the RBS B0030. Ran E-gel to check PCR. Cut osmY plasmid with Spe, and RBS B0030 with Xba, and ligated the two. Transformed cells with this construct. Then realised this was silly.