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Competence test

  • Transformed PUK plasmid and YFP plasmid into mutant E.coli strains.

osmY and Kdp

  • digested PCR products, and death gene vector with EcoR1 and Pst1
  • ligated new BioBricks into vector
  • Transformed into competent TOP10 cells
  • Plate out onto Chloramphenicol plates