IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/13
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Grow RBS S6- Put the 10mL of LB with RBS S6 from yesterday into 200mL of LB with antibiotic - Incubate at 37°C with shaking RBS W screening- Plasmid miniprep of RBS W2, 4, 6, 8, 10 9witouh endo wash buffer) - Nanodrop
- Single digest with EcoRI and XbaI - Gel1
- W2 with EcoRI : cut - W2 with XbaI : uncut, self ligation - W4 with EcoRI : cut - W4 with XbaI : uncut, self ligation - W5 with EcoRI : cut - W5 with XbaI : uncut, self ligation - W6 with EcoRI : cut - W6 with XbaI : cut (2 bands), transformation ok for RBS W6 - W8 with EcoRI : impossible to see - W8 with XbaI : impossible to see - W10 with EcoRI : cut - W10 with XbaI : maybe cut, it seems to be 2 bands, but quite difficult to see - W11 with EcoRI : cut - W11 with XbaI : uncut, self ligation Double check of RBS W6 and stock- Grow RBS W6 in 10mL of LB with antibiotic |