IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/08

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Check product of PCR from yesterday

  • Lane3 : Hyperladder I
  • Lane4 : agrA
  • Lane5 : agrB
  • Lane6 : Pxyl
  • Lane7 : Pspac
  • Lane8 : Ppac
  • Lane9 : Pupp
  • Lane10 Hyperladder IV
  • Results : everything is ok

Check transformation from 03/09

After several days of incubation, we have a few colonies on our transformed plates (agrA, B and C).

  • Single colony PCR

- Add 13μL of cells diluted into SDW, 5μL of MM, 1μL of VF and 1μL of VR

  • Gel