IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/28

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Results from transformation of ligation products

- nothing on plate, even on control plate

  • Reasons ?

- cells non competent anymore (try with fresh competent cells)

- not enough DNA (try with 10μL of DNA)

- very short ligation products

Transformation of ligation products (new)

  • Products to ligate : Pupp, Pspac, agrD, RBS S and RBS W

- new fresh competent TOP 10

- 5μL of DNA (1.5μL of PUC9)

- 2h30 in the incubator