IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/06

From OpenWetWare

Signalling button.gif
Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

PCR parts of Agr operon

We received primers for Agr A,B,C, and D today - http://openwetware.org/wiki/IGEM:Cambridge/2008/Turing_Pattern_Formation/Primers

We must PCR each individual part out of the previous BioBricks (I746001 &I746101) in order to put our bacillus RBS on them.

I was concerned that the region of most homology would be the BioBrick prefix and suffix itself, so I first digested the DNA with Xbal and SpeI.

For some reason the digestion didn't work that well, so I've decided to go ahead and use the uncut BioBrick DNA as template for my reactions.