IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/07/29

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Biobrick Handling...

  • I0500 promoter and pSB3K3 plasmid PCR-ed following previous protocol
  • 0.8% agarose gel with SyBr Green used
    • Lane 4 - Hyperladder I
    • Lane 5 - I0500
    • Lane 6 - I0500 cut with SpeI
    • Lane 8 - pSB3K3
    • Lane 9 - pSB3K3 cut with EcoRI and PstI

Result: Unknown band shown on lane 8 with a much smaller size (400bp) than expected. Faint band on lane 6 (1200bp) corresponding to I0500 gene size. Other lanes didn't work.