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Result for Bacillus transformation (27/08)

Strain Vector Antibiotic number of colonies Conclusion
IA751 None Spc50 0 ok
IA751 ECE153 Spc50 about 200 very good efficiency
IA751 None Blank confluent cells are alive
IA751 None Cm5 a lot problem of contamination (bacillus??? or E.coli???)
IA751 ECE112 Cm5 a lot to check

New Bacillus transformation (to make glycerol stocks)

  • 2 attempts with plates from 12/08 and 18/08, no growth... Cells may be dead.
  • New plate of IA771 and IA751 (with glycerol stocks)