IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/08/21

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Miniprep

  • Minipreps of successful constructs identified on Aug 19 were performed using Raf's Miniprep protocol
  • The constructs were:
  • pSB1C3 + LoxR
  • pSB1A3 + LoxR
  • pSB1C3 + Cre-LVA
  • pSB1A3 + Cre-LVA
  • Plasmids were resuspended to 100ng/μL for sequencing
  • note: sequences verified Aug 24 - 26 (good sequencing results by another teammate)