IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/07/27

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Restriction double digest of Lock/Key/Control lock

  • standard UBC iGEM protocols and calculations, with EcoRI and PstU
  • ran results on gel - no correct band present; redo

sequencing

sent in BBa_J31001 (Hin-LVa) for sequencing

Results of July 24 transformation

plates:

  • ASEM 1, 2, 9: no colony
  • ASEM 10, 11, 12: defunct -> threw away
  • ASEM 6 and c1: colonies (~5) present

Innoculation

innoculated all 3 colonies of ASEM 16 into LB-Chlor (5mL) while plating onto a spot plate