IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/26

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Testing Competent Cells

  • In order to test the competence of the DH5α cells, one glycerol stock from each of the four competent cell tubes (A,B,C & D) were made using:
    • 14uL 100% Glycerol
    • 86uL Competent cells
  • Each 100uL aliquot was put into the -80 freezer for 2 hours
  • The 4 aliquots of competent cells were transformed using 1uL of pUC19 following the iGEM:British Columbia Transformation protocol
  • In addition, one aliquot of DB3.1 cells that have been previously tested to be competent were also transformed with the same conditions as a control