IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/24

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Miniprep of pBAD Strong and pBAD weak+RFP

  • Used an Invitrogen ChargeSwitch Miniprep plasmid kit to perform the miniprep
  • As the cells were grown overnight at 30°C instead of 37°C, there was a lower yield then expected. As a result, the cells were resuspended in 50uL of buffer instead of 100uL to raise the concentration