IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/09

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Efficiency of 09/06/01 DH5α Cells

  • 34 colonies were counted when 500uL of cells were plated onto a LB-Amp plate
  • 2uL of 10pg/uL pUC19 plasmid was added to the competent cells
  • Calculations give 1.7x10^6 transformants/ ug