IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/07

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Overnight Cultures

  • Prepared 4 overnight cultures in order to make competent cells of BW27783 and DH5α and to miniprep the chlor and amp construction plasmid
  • Innoculated 5mL of LB broth with 09/05/13 BW27783 cells
  • Innoculatd 5mL of LB broth with 09/05/13 DH5α cells
  • Innoculated 5mL of LB-amp broth from a plate of DB3.1 cells containing the Amp construction plasmid
  • Innoculated 5mL of LB-chlor broth from a plate of DB3.1 cells containing the chlor construction plasmid
  • All cultures were grown overnight in a shaking incubator at 32°C