IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/05/16

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Making Competent Cells

  • Had no access to a -80°C freezer, so stored everything at -20°C.
  • Added 0.42mL 100% glycerol to cells (~3mL), aliquoted 200uL per tube.
  • Stored at -20°C at 4:00PM.