IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/05/15

From OpenWetWare
Jump to: navigation, search
Igem-logo-150px.png iGEM Project name 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Competent Cell Preparation

  • (for each strain) Discovered that the shaking incubator had crept up to 37°C overnight. Diluted 0.45mL of culture into 50mL LB medium. Incubated at 30°C in shaking incubator, starting at 8:50AM.
  • Checked OD600nm at 2:50PM: DH5a 0.6 0.8 (used as 0.7) DB3.1 1.8 1.3 (used as 1.8) --> diluted to 0.4 in 40mL LB.
  • Followed competent cell protocol exactly as written.
  • Used non-sterile centrifuge bottles, so there is some risk of contamination.
  • Stored cells at 4°C at 6:36PM.