IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/05/14

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Freezer Stocks - Streaked Plates

  • Culture from freezer stocks grew well. Our streaking still needs a bit of improvement.
  • Plates were sealed with parafilm and stored at 4ºC.

Competent Cell Preparation

  • Inoculated 5mL LB from streaked glycerol stocks of DB3.1 and DH5α (streaked 090513) at 3:25PM. Left in shaking incubator overnight at 30°C.