IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/09/09

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9/9

Ethanol
Started shake flasks of seeds for RCA

  • Added 500 μL cells to 50 ml LB with 50 μL of chloramphenicol
  • let grow to O.D. of about .7
  • induced with 25 μL IPTG (.5mM)
  • let grow overnight