IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/29

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Tuesday 7/29

  • Miniprepped the lacI and the TesA+ RBS. There was a lot of cells with both.
  • Did gel of both, and those did not turn out well. The LacI was streaked due to contamination and degradation and the the TesA + RBS was too close to the well so there was probably error in either the miniprep or the digestions of them. TesA+ RBS was digested at the x and p sites while the lacI was digested at the s and p sites.

Had to restart those. Did the seeds of both, and re-streaked the TesA +RBS from plate 4, colony 3.

Reagent Volume Lanes+Length:
1. 1 kb Ladder
3. LacI = 2000
5. TesA+RBS = 650
DNA (LacI plasmid) 10.0 μL
10X buffer 2.0
PstI 1.0
SpeI 1.0
dH2O 6.0
  20.0 μL --> 37°C/ ~1 Hr.