IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/28

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Monday 7/28

  • Started a seed of the seed of colony #3 from ligation plate #4.
  • Started a seed of the LacI promotor part (R0011) from Kylie

Set up a digestion of RBS+TesA to be run on a gel tomorrow

Reagent (μL) Volume (μL)
DNA(RBS+TesA plasmid) 10.0 μL
10X fast digest buffer 2.0
dH2O 6.0
XbaI 1.0
PstI 1.0
  20.0 μL --> 37°C/ ~overnight