IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/21

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7/21

  • Ligations started for TES with RBS and control. Following are the tables.
Reagent (μL) Volume (μL)
DNA(plasmid) 1.0 μL RBS, 2.0 μL TesA
10X buffer 1.0
dH2O 5.0
Ligaze 1.0
  10.0 μL --> 16°C/ ~overnight
Reagent (μL) Volume (μL)
DNA(plasmid) 1.0 μL RBS, 0 μL TesA
10X buffer 1.0
dH2O 7.0
Ligaze 1.0
  10.0 μL --> 16°C/ overnight
  • Transformations of Tet (Tube 4) on competency cells.
  • 50μL of cells with 1μL of Tet, 50μL with 5μL of Tet, and one with 50μL cells with 10μL of Tet. Plated these cells on chloramphenicol plates.