IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/07/02

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Ligation Reactions

  • Began design, but did not begin reaction
  • Independently digest the TesA (sample 1), RBS (sample 7) to combine with provided backbone (24KS EPKS)which has been cut at the E and P sites.

TesA

  • Digest 5 μL DNA with 1 μL buffer, 1μL Xba1, 1 μL Pst1, and 2μL of water.

RBS

  • Digest 5 μL DNA with 1 μL buffer, 1μL EcoR1, 1 μL Spe1, and 2μL of water.

Ligation

Transformation