IGEM:American University/2009/Notebook/BCHM 2/2017/02/08
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The solutions used in the scans today were prepared as described below.
The solutions above were not prepared as the article described. We did not thioacetate in the preparation of the soln. Because there was no sulfur source in the soln, no quantum dots were formed. The peak that we would expect from the quantum dots would be in the 400-600 nm region, and we can see from the graph below that there is no major peak. The large peak around 650 nm is an overtone.
We can see from the selected view of the scan that there is some broad activity in the 400-600 nm region. This may be a scan of fluorescent residues in myoglobin. To confirm this, we will do a background scan of only myoglobin next week.