Haynes Lab:Notebook:Shared/Cloning Bootcamp/2015/04/29

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Haynes Lab Cloning Bootcamp Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Daniel Vargas - 04/29/2015

mCherry pSB1A3 using Amp / DH5α-Turbo

Inoculation 04/28/15 http://openwetware.org/wiki/Haynes_Lab:Notebook:Shared/Cloning_Bootcamp/2015/04/28

Liquid culture 1: growth

Liquid culture 2: growth

Streak 1: growth

Streak 2: growth

Minipreps

Sample 1: mCherry pSB1A3 colony 1; 2H5Q; part 748bp; vector 2155

Sample 2: mCherry pSB1A3 colony 2; 2H5Q; part 748bp; vector 2155

DNA Concentration Data

Plasmid OD260 OD260/280 ng/μL
1. Plasmid 1 0.197 1.888 197.489
2. Plasmid 2 .130 1.889 129.815


Restriction Digest Table

  • Checked plasmid minipreps with EcoRI/PstI digests
Reagent Volume Expected:
1. Plasmid 1 = 746, 2155
2. Plasmid 2 = 746, 2155
Today's gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 3.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 8.5
  15 μL --> 37°C/ 15 min.