Haynes Lab:Notebook:Shared/Cloning Bootcamp/2015/03/05

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Mara Peterson - 03/05/15

Plasmid Verification- Finding Concentrations and Mini Prep

Antibiotic Resistance Gene: Ampicillin

(1) Sample 1, Colony 1: Plasmid DNA- KAH158/MV2, length: 7264 bp, vector length: 4529 bp

(2) Sample 1, Colony 2: Plasmid DNA- KAH158/MV2, length: 7264 bp, vector length: 4529 bp

(3) Sample 2, Colony 1: Plasmid DNA- KAH228/MV1, length: 7463 bp, vector length: 5428 bp

(4) Sample 2, Colony 2: Plasmid DNA- KAH228/MV1, length: 7463 bp, vector length: 5428 bp

  • The pelleted DNA was resuspended and measured for concentrations.
Sample Replicate 1 ng/µL Replicate 2 ng/µL
1 194.854 173.005
2 112.857 112.79
3 355.235 322.202
4 55.171 51.958
  • The Mini Prep kit was used to prepare the samples for DNA elution and Gel Electrophoresis (completed on the same day by Shawn Striker).