Haynes Lab:Notebook/TB Biosensor/2012/06/28
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digested LuxS and LsrA minipreps from 6.27.12, crtE,I,B miniprep from 6.21.12 and Dr. Haynes' V0120 plasmid with Xba1 and Pst1
ran on a gel, cut out, extracted
made chloramphenicol stocks in ethanol at 20mg/ml.
Ernesto and I poured Cmp and Kan LB plates.
I told Ernesto to cut the wrong bands (I think he cut out the backbone!)
I picked more colonies from RBS+LuxS and LsrA, put into 2ml of LB+amp, shook overnight