Haynes Lab:Notebook/TB Biosensor/2012/06/15

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ligations from 12.06.14:

crtE (3) + V0120 : no colonies

crtI (8) + V0120: no colonies

V0120 backbone plate: one colony

Behzad's V0120 vector #1: many colonies

nothing: no colonies

Put plates at 4deg.

Decided to try Dr. Haynes' KAH40 for V0120 backbone.

Cut KAH40 with Pst1 and Xba1 for 30 min at 36deg. Put at -20deg.