Haynes Lab:Notebook/Synthetic Chromatin for Cancer Research/2015/11/05

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11-5-15

  • Not enough DNA was used in the previous digest.
  • Digest pet28, DT017, DT028, DT040
    • Digest using Bam and XhoI
    • Aim for about 700ng of PcTF and 350ng of pet28.
  • PCR clean up for backbone and the three inserts.
  • Ligated the three inserts with the backbone. Used the classic ligation for beginners protocol.