Haynes Lab:Notebook/Synthetic Biology and Bioinformatics for Predictable Control of Therapeutic Gene2/2012/08/27

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Plate Reading EB buffer is your blank Use Gen5, Nucleic Acid Quantification, dsDNA sample We are going to use a Take3 plate with a blank at the top right corner and samples below Wash well plate 2uL of buffer, 2uL of sample, 2 uL of sample, etc (Move fast, liquid is prone to evaporation) Close lid gently! Open slide holder (make sure power is on) and place Take3 plate Click read Click approve is it says "valid" Your ratio should be between 1.8 and 2.0 Record values for 260, 260/280, and ng/uL Sample 1: 260: 0.179 260/280: 1.902 ng/uL: 179.081 Sample 2: 260: 0.053 260/280: 1.912 ng/uL: 52.884 (this value is low, sample 2 isn't as desirable)