Haynes Lab:Notebook/Short Projects/2015/06/11

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Ben Nyer - 11/06/15

Cloning gRNAs into pSPgRNA for dCas9-HT binding to luc gene

Sequencing results came back for g031_pSPgRNA plasmid. Colony 1 contains errors in the insert region, but colony 2 is a match. All four gRNAs are in pSPgRNA and stored in 'Karmella Box 2' in the -20 °C freezer.