Haynes Lab:Notebook/Short Projects/2014/09/26

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09/26/14 - Meli'sa Crawford

  • Plasmid DNA Sequencing Results

Plasmid DNA Sequencing Results:

  • Analyzed the results from the DNA sequencing done for plasmid DNA BD003, BD004, BD005 and BD006.
  • BD006 was a match for the construct D11.12
  • We can conclude that the plasmids have the V0120 backbone.
  • Next steps: Design gRNA sequences for CRISPR. Want to utilize CRISPR complex to assist in inserting D11.12 into FRT landing pad region.