Haynes Lab:Notebook/Short Projects/2014/09/21

Haynes Lab - Short Projects

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09/21/14 - Meli'sa Crawford Restriction Digest of Plasmid DNA Restriction Digest 1. 60ml of 1x-TAE 2. 6 grams Argarose (Low EEO) 3. Restriction Enzymes: EcoRi and PSTL 4. Buffer: FD Green Buffer 5. 6.0μL of DNA cell stain 6. 5.0μLof plasmid DNA 7. 6.5μL of H2O Chose the following plasmid DNA: BD003, BD004, BD005, BD006 We know that the length of BD005 is 2422 bp DPRE also known as D11.12 is 1740bp We assume that BD006 must have the D11.12 construct. If this is so, the length of BD006 would be 4126bp Results: BD005 and BD006 seemed to be the same length. This could mean that BD006 could be the same plasmid DNA May need to perform new restriction digest. Buffer may have been too diluted. However, this couldn't effect the outcome of size.