Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2016/08/16
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Repeated dox dose curve with 6-well plates (see previous days). Samples are 1.1-1.3 (1 µg/mL dox), 6.1-6.3 (31 ng/mL dox), 7.1-7.3 (16 ng/mL dox), and 8.1-8.3 (no dox).
Harvested cells with TRIzol and extracted RNA using RNeasy mini kit. Concentrations/calculations for cDNA synthesis:
Following steps from cDNA synthesis protocol.