Haynes Lab:Notebook/RNA-seq of PcTF Transfected U2OS & SK-N-SH cell lines/2016/08/04

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SKNSH KAH132 Transient Transfection Results - Microscopy

24 hours

Well 1:
2016-08-02 Well 1 KAH132-MV2 phase mCherry 10x.jpg


Well 2:
2016-08-02 Well 2 KAH132-MV2 phase mCherry 10x.jpg


Well 3:
2016-08-02 Well 3 KAH132-MV2 phase mCherry 10x.jpg


Well 4:
2016-08-02 Well 4 KAH132-MV2 phase mCherry 10x.jpg


Well 5:
2016-08-02 Well 5 KAH132-MV2 phase mCherry 10x.jpg


Well 6:
2016-08-02 Well 6 KAH132-MV2 phase mCherry 10x.jpg



48 hours


Well 2:
2016-08-03 Well 2 KAH132-MV2 phase mCherry 10x.jpg


Well 3:
2016-08-03 Well 3 KAH132-MV2 phase mCherry 10x.jpg


Well 5:
2016-08-03 Well 5 KAH132-MV2 phase mCherry 10x.jpg


Well 6:
2016-08-03 Well 6 KAH132-MV2 phase mCherry 10x.jpg



72 hours


Well 3:
2016-08-04 Well 3 KAH132-MV2 phase mCherry 10x.jpg


Well 6:
2016-08-04 Well 6 KAH132-MV2 phase mCherry 10x.jpg


SKNSH KAH132 Transient Transfection Results - Flow Cytometry

24hr:

Screen Shot 2016-09-20 at 10.35.33 AM.png


48hr:

Screen Shot 2016-09-20 at 10.35.41 AM.png

72hr:

Screen Shot 2016-09-20 at 10.35.46 AM.png

Sample ID % RFP
24hr A 36.2
24hr B 39.2
48hr A 34.2
48hr B 38.5
72hr A 22.8
72hr B 21.6